Figure 6.

An increase in circulating EPCs was seen in response to DMOG in WT mice but not in HIF-1α KO mice. Scatterplot of flow cytometry fluorescence intensity isolates percentages of peripheral blood leukocytes that were double-positive for Sca1/cKit (A and C) and the histogram of triple-positive for Sca1/cKit/VEGFR2 (B and D). There was a significant increase in circulating EPCs in response to DMOG in WT mice; however, there was no such response seen in the HIF-1α KO mice (^∗∗∗P < 0.001). E: Overall model of how the liver can be stimulated to act as an endocrine organ to protect distal capillary beds such as in the eye and specifically the retina. Data are expressed as means ± SEM (C and D). n = 6 mice per group (C and D). APC, allophyocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin.