Table 2.
Sensitivity and specificity of multiplex SONIA assay using dried blood spot specimens compared to live virus assays (N = 160).
| Live virus assay positive | Multiplex SONIA assay positive | Agreement rate | ||
|---|---|---|---|---|
| Vaccinated group (N = 40) | Wild type | 40 | 39 | 39/40 (98%) |
| Delta | 34 | 31 | 31/34 (91%) | |
| Alpha | — | 38 | — | |
| Live virus assay negative | Multiplex SONIA assay negative | Agreement Rate | ||
| Healthy control group (N = 80) | Wild type | 80 | 80 | 80/80 (100%) |
| Delta | 80 | 80 | 80/80 (100%) | |
| Alpha | 80 | 80 | 80/80 (100%) | |
| Lyme disease group (N = 40) | Wild type | 40 | 40 | 40/40 (100%) |
| Delta | 40 | 40 | 40/40 (100%) | |
| Alpha | 40 | 40 | 40/40 (100%) |
Matched serum and dried blood spot samples were obtained from the participants. Multiplex SONIA assay was performed on the dried blood spot specimens, and the live virus assays were performed for the wild type and delta strain for the vaccinated group.