KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC anti-mouse CD45 | Biolegend | Cat# 103,111; RRID:AB_312976 |
| FITC anti-mouse TER119 antibody | Biolegend | Cat# 116,206; RRID:AB_313707 |
| PerCP/Cy5.5 anti-mouse Ly-6A/E (Sca-1) | Biolegend | Cat# 108124; RRID:AB_893615 |
| APC/Cyanine7 anti-mouse CD117 | Biolegend | Cat# 105826; RRID:AB_1626278 |
| PE anti-mouse CD41 antibody | Biolegend | Cat# 133906; RRID:AB_2129745 |
| PE/Cy7 anti-mouse CD150 | Biolegend | Cat# 115914; RRID:AB_439797 |
| FITC anti-mouse CD16/32 antibody | Biolegend | Cat# 101305; RRID:AB_312804 |
| APC anti-mouse CD105 antibody | Biolegend | Cat# 120414; RRID:AB_2277914 |
| PE anti-mouse/human CD44 | Biolegend | Cat# 103008; RRID:AB_312959 |
| PE anti-mouse CD71 antibody | Biolegend | Cat# 113807; RRID:AB_313568 |
| PerCP/Cy5.5 anti-mouse TER-119 | Biolegend | Cat# 116228; RRID:AB_893636 |
| Rabbit anti-CHD8 Antibody | Bethyl | Cat# A301–224A; RRID:AB_890578 |
| RhoA (67B9) Rabbit mAb antibody | Cell Signaling Technology | Cat# 2117; RRID:AB_10693922 |
| Anti-Rac1, clone 23A8 antibody | Millipore | Cat# 05–389; RRID:AB_309712 |
| Cdc42 (11A11) Rabbit mAb antibody | Cell Signaling Technology | Cat# 2466; RRID:AB_2078082 |
| Phospho-Myosin Light Chain 2 (Ser19) Antibody | Cell Signaling Technology | Cat# 3671; RRID:AB_330248 |
| Myosin Light Chain 2 (D18E2) antibody | Cell Signaling Technology | Cat# 8505; RRID:AB_2728760 |
| Chemicals, peptides, and recombinant proteins | ||
| 4’,6 Diamidino 2 Phenylindole, Dihydrochloride | Thermo Fisher Scientific | Cat# D1306 |
| Propidium Iodide Solution | BioLegend | Cat# 421301 |
| Methylcellulose-based medium with EPO (without other cytokines) for mouse cells | STEMCELL Technologies | Cat # 03334 |
| Serum-free methylcellulose-based medium with recombinant cytokines (including EPO) for mouse erythroid progenitor cells | STEMCELL Technologies | Cat # 03436 |
| Harleco® Hematology Stains and Reagents | Sigma-Aldrich | Cat # 740–75 |
| Critical commercial assays | ||
| FITC BrdU Flow Kit | BD Biosciences | Cat # 559619 |
| FITC Annexin V 100 tests | BioLegend | Cat # 640906 |
| Mouse Erythropoietin ELISA Kit | Abcam | Cat # ab270893 |
| Camco Stain Pak-3 bottle stain kit | Cambridge Diagnostic Products | Cat # 702 |
| CUT&RUN Assay Kit | Cell Signaling Technology | Cat # 86652S |
| NEBNext Ultra II DNA Library Prep with Sample Purification Beads | New England Biolabs (NEB) | Cat # E7103S |
| NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 4) | New England Biolabs (NEB) | Cat # E7730S |
| Miltenyi Biotec, Inc. CD45 MICROBEADS | Miltenyi Biotec | Cat # 130-052-301 |
| Miltenyi Biotec, Inc. TER 119 MICRO BEADS MOUSE | Miltenyi Biotec | Cat #130-049-901 |
| Deposited data | ||
| ProE RNA sequencing | This paper | GSE: GSE189853 |
| CHD8 CUT&RUN sequencing data | This paper | GSE: GSE189853 |
| Experimental models: Organisms/strains | ||
| Chd8F/F mice | Q. Richard Lu lab | Zhao et al., 2018 |
| Trp53F/F mice | THE JACKSON LABORATORY | Cat # 008462 |
| Mx1-Cre mice | Yi Zheng lab | N/A |
| Oligonucleotides | ||
| Primers for Real-time PCR, see Table S1 | This paper | N/A |
| Software and algorithms | ||
| GraphPad Prism 8.4.0 | GraphPad | https://www.graphpad.com/ |
| FlowJo V10 | BD (Becton, Dickinson & Company) | https://www.flowjo.com/bd-flowjo-faq |
| FACS Diva | BD Biosciences | https://www.bdbiosciences.com/en-it/products/software/instrument-software/bd-facsdiva-software |
| DESeq2 | Bioconductor | https://doi.org/10.18129/B9.bioc.DESeq2 |
| EnhancedVolcano R package | Bioconductor | https://doi.org/10.18129/B9.bioc.EnhancedVolcano |
| pheatmap R package | RDocumentation | https://rdocumentation.org/packages/pheatmap/versions/1.0.12 |
| Metascape web tools | Zhou et al., 2019 | https://metascape.org/gp/index.html#/main/step1 |
| SEACR 1.4.R | Meers, MP, Tenenbaum, D and Henikoff S (2019) | https://seacr.fredhutch.org/ |
| IGV 2.8.2 | Helga Thorvaldsdόttir, James T. Robinson, Jill P. Mesirov. (2013) | https://igv.org/ |
| Image Lab software | Bio Rad | https://www.bio-rad.com/zh-cn/product/image-lab-software?ID=KRE6P5E8Z |