TABLE 4.
EE of 1,4-disubstituted cis-benzene dihydrodiols formed by several dioxygenases
Substrate | EE (%) of the dioxygenation product formed by:
|
||
---|---|---|---|
P. putida UV4 TDOa | P. putida F39/D TDO | CDOb | |
4-Fluorotoluene | 83 | 90 | 49 |
4-Chlorotoluene | 15 | 10 | 77c |
4-Bromotoluene | 37 | 26 | 77 |
4-Iodotoluene | 80 | 80 | 98 |
1-Chloro-4-iodobenzene | 15 | NDd | 67 |
Data taken from reference 3.
Pseudomonas sp. strain P51 CDO, cloned into E. coli DH5α(pTCB 144).
EE of the major isomer.
ND, not determined.