TABLE 5.
Products formed from various aromatic substrates by whole cells of E. coli DH 5α(pTCB144), which contains the CDO of Pseudomonas sp. strain P51
| Substrate | Relative amt (mol%)a | Adsorbance maxima (nm)b | m/z of prominent ions, (relative abundance)c | Suggested structure |
|---|---|---|---|---|
| Dibenzofuran | 88 | 303 | 268, M+ (100); 184 (18); 156 (17) | cis-1,2-Dihydroxy-1,2-dihydrodibenzofuran |
| 12 | ND | 268, M+ (100); 184 (26); 168 (21) | cis-3,4-Dihydroxy-3,4-dihydrodibenzofuran | |
| 3-Chlorodibenzofuran | 100 | ND | 304, M+ +2 (33); 302, M+ (100); 245 (13); 218 (99); 202 (46); 190 (46) | Chloro-cis-dihydroxydrodibenzofurand |
| Dibenzodioxin | 100 | 293 | 284, M+ (90); 227 (12); 200 (78); 184 (100); 172 (28); 156 (12); 128 (36) | cis-Dihydroxydihydrodibenzodioxind |
| Diphenylether | 90 | 278 | 270, M+ (100); 213 (27); 186 (55); 170 (42); 158 (23); 141 (36); 120 (65); 77 (90); 65 (92); 51 (78) | cis-Dihydroxydihydrodiphenyletherd |
| 10 | ND | 270, M+ (80); 186 (100); 86 (35); 77 (30) |
a
Relative to the total amount of products, calculated by integration of GC-MS chromatograms (TIC, m/z = 90 to 400).
b
By HPLC. ND, not determined.
c
The m/z values of the prominent ions of the mass spectra of the BB derivatives are given. Relative abundance, in parenheses, is given as a percentage.
d
Exact isomeric structure unknown.