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. 2022 Apr 29;21(16):1697–1709. doi: 10.1080/15384101.2022.2067438

Figure 3.

Figure 3.

MiR-497-5p directly targeting CX3CL1 gene. (a) The sequence of miR-497-5p binding sites with WT/MUT CX3CL 3′-UTR. (b) Relative luciferase reporter activities of WT/MUT CX3CL1 in HEK293 cells. (c) Expression of CX3CL1 in NSCLC tissues (n = 30) and adjacent normal tissues (n = 30) was measured by RT-qPCR. (d) RT-qPCR and (e) Western blot analysis was performed to determine the expression of CX3CL1 in different cell lines, including A549, H460, H226 and H1299 and BEAS-2B. (f) RT-qPCR and (g) Western blotting analysis were performed to determine expression of CX3CL in A549 or H460 cells transfected with miR-497-5p mimic, miR-497-5p inhibitor, or their negative controls. Values are expressed as means ± SEM, n = 5. * P < 0.05 and ** P < 0.01.