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. 2022 Apr 29;21(16):1697–1709. doi: 10.1080/15384101.2022.2067438

Figure 4.

Figure 4.

Knockdown of CX3CL1 suppressed the cell proliferation and invasion in A549 and H460 NSCLC cells. A549 and H460 cells were respectively transfected with 1 µg/mL lentiviral interference vector against CX3CL1 (CX3CL1 shRNA) and negative control vector (scrambled shRNA, scramble). Following transfection for 48 h, (a) Expression of CX3CL1 was measured by RT-qPCR in A549 and H460 cells. GAPDH was used as an internal reference. (b) Cell proliferation was examined using the CCK-8 assay in A549 and H460 cells. (c) Cell apoptosis was detected by flow cytometry. (d) Cell invasion was examined by Transwell assay. n = 5. **P < 0.01.