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. 2022 Apr 29;21(16):1697–1709. doi: 10.1080/15384101.2022.2067438

Figure 5.

Figure 5.

CX3CL1 rescued the suppression of miR-497-5p on phosphorylation of AKT and ERK in A549 cells. Human NSCLC A549 cells were respectively transfected with 40 nM miR-497-5p mimic alone or together with 1.2 µg/mL pcDNA vector expressing CX3CL1 (pcDNA-CX3CL1). Following transfection for 48 h, (a) The phosphorylation of AKT and ERK was observed by Western blotting analysis. (b) RT-qPCR was performed to determine expression of MMP-2 and MMP-9 mRNA in A549 NSCLC cells. (c) Western blotting was performed to determine expression of MMP-2 and MMP-9 protein in NSCLC cells. Quantification of band intensity was performed by ImageJ software, normalized to GAPDH. Values are expressed as means ± SEM, n = 5. * P < 0.05 and **P < 0.01 vs. negative control group (NC mimic + pcDNA3.1).