Figure 6.

MiR-497-5p suppressed the phosphorylation of ERK and AKT. A549 or H460 cells were treated with miR-497-5p mimic (40 nM) or together with ERK inhibitor U0126 (10 µM) for 48 h. (a and b) Expression of miR-497-5p was detected with RT-qPCR. (c-g) The phosphorylation of AKT and ERK was observed by Western blotting analysis. Quantification of band intensity was quantified by ImageJ software, normalized to GAPDH. Statistical significance was determined with one-way ANOVA test. Values are expressed as means ± SEM, n = 5. * P < 0.05 and ** P < 0.01 vs. NC inhibitor.