Figure 4.

a) A representative agarose gel (0.8% w/v) of resolved RT-PCR products from Col-0 WT and the PDI9 overexpressor (OE) plants following amplification of the transcripts for the PDI9 gene, the bZIP60 locus (total RNA = bZIP60t, and spliced RNA = bZIP60s), and the house keeping gene Actin in response to 2 mM DTT treatment in leaf protoplasts. b) Measurement of the RNA levels for the PDI9 gene and the bZIP60 locus (total RNA = bZIP60t, and spliced RNA = bZIP60s) in response to 2 mM DTT treatment in leaf protoplasts from Col WT and the OE plants. RT-PCR was conducted and the resulting bands were quantitated relative to actin mRNA controls. The means ± standard deviations are represented from triplicate experiments. **p < .01 and *p < .05, showing statistical difference from one-way ANOVA analysis with post-hoc Tukey HSD Test.