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. 2022 Apr 7;79:10067. doi: 10.3389/bjbs.2022.10067

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Copyright © 2022 Zheng, Xiao, Zhang and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SLIT1 was upregulated in glioma and was associated with radiation resistance. (A) Quantitative real-time (qRT)-PCR was carried out to determine the expressions of SLIT1 in glioma tissues and matched adjacent normal tissues. (B) Western blotting was carried out to determine the protein expressions of SLIT1 in glioma tissues and matched adjacent normal tissues. (C) The expressions of SLIT1 in glioma cells (U251 and A172) and normal human astrocytes NHA cell line were evaluated by qRT-PCR. (D) The expressions of SLIT1 in glioma cells were examined by qRT-PCR within 24 h after 0, 2, 4, 6, 8 Gy irradiation. (E) The expression of SLIT1 in U251 and A172 cells transfected with Si-SLIT1 was examined by qRT-PCR. (F) The protein expression of SLIT1 in U251 and A172 cells transfected with Si-SLIT1 was measured by western blot. *p < 0.05; **p < 0.001.