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. 2022 Apr 7;79:10067. doi: 10.3389/bjbs.2022.10067

FIGURE 4.

FIGURE 4

Downregulation of miR-640 enhanced radiosensitivity of glioma cells and inhibited tumor progression by promoting SLIT1 expression. (A) The expression of SLIT1 in U251 and A172 cells transfected with miR-640 inhibitor or Si-SLIT1 was determined using qRT-PCR. (B) The protein expression of SLIT1 in U251 and A172 cells transfected with miR-640 inhibitor or Si-SLIT1 was determined using Western blot. (C) Transfected U251 and A172 cells were exposed to different doses of irradiation, followed by cell viability evaluation by CCK-8 assay. (D) U251 and A172 cells with miR-640 inhibitor or Si-SLIT1 transfection were subjected to 4 Gy irradiation, followed by the measurement of cells migration rate by wound healing assay. (E) The transfected U251 and A172 cells were treated with irradiation at dose of 4 Gy, followed by the measurement of transwell invasion assay. (F) Caspase-3 activity was measured in transfected U251 and A172 cells after 4 Gy irradiation treatment. *p < 0.05, **p < 0.001 vs. 4 Gy + inhibitor-NC/si-NC. # p < 0.05, ## p < 0.001 vs. 4 Gy + si-SLIT1+inhibitor.