. Author manuscript; available in PMC: 2022 Nov 1.

Published in final edited form as: J Chromatogr Open. 2022 Jun 3;2:100054. doi: 10.1016/j.jcoa.2022.100054

Table 3.

Stability of thiotepa, tepa, CP, and 4OHCP (n=3).

Conditions	Untreated ng/mL	Treated ng/mL	Remaining %	Untreated ng/mL	Treated ng/mL	Remaining %
	Thiotepa			Tepa
−70 °C, 34 months	2.85±0.24	2.81±0.29	98.6	3.00	3.25±0.33	108
−70 °C, 34 months	1560±150	1570±60	101	1440±80	1590±30	110
22±3 °C, 60 hr	3.01±0.11	3.08±0.25	102	2.76±0.02	3.18±0.07	115
	1570±60	1370±60	87.3	1740±90	1550±20	89.1
80 hr		2.87±0.09	95.3		3.22±0.22	117
		1300±40	82.8		1550±60	89.1
4 °C, 11 days	3.01±0.11	3.14±0.21	104	2.76±0.02	2.95±0.22	107
	1570±60	1380±50	87.9	1740±90	1590±70	91.4
Freeze-thaw 4-cycles	3.01±0.11	3.42±0.10	114	2.76±0.02	3.23±0.16	117
Freeze-thaw 4-cycles	1570±60	1550±40	98.7	1740±90	1780±50	102
Autosampler 3 days	3.37±0.13	3.43±0.12	102	2.96±0.09	3.20±0.06	108
Autosampler 3 days	1590±60	1670±70	105	1630±60	1770±50	109
Stock solution
−70 °C, 31 months	(428±8)×10³	(421±10)×10³	98.4	(148±3)×10⁴	(158±6)×10⁴	106
22±3 °C, 22hr	(651±9)×10³	(663±6)×10³	102	(911±18)×10³	(980±30)×10³	108
	CP			4OHCP
−70 °C, 42 days	63.1±2.3	68.4±3.9	108	61.0±2.3	64.2±3.5	105
	8030±120	8490±530	106	4060±130	4390±200	108
22±3 °C, 24hr	67.5±3.5	64.8±5.3	96.0	65.6±6.9	62.7±4.8	95.6
	7420±430	8330±480	112	4200±290	4380±280	104
Freeze-thaw 3 cycles	64.8±4.1	65.5±5.5	101	61.4±2.6	69.7±0.2	114
Freeze-thaw 3 cycles	8310±250	8140±230	98.0	4190±240	4190±150	100
Autosampler 80hr		66.4±2.4	111		64.6±8.9	108
Autosampler 80hr		8600±140	108		3930±220	98.3
Stock solution
−70 °C, 2 months				(106±7)× 10⁴	(98.9±5.3)×10⁴	93.3
−70 °C,16 months	(297±3)×10⁴	(300±2)×10⁴	101

%remaining was calculated by comparing to the untreated values (100%).