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. 2022 Jul 8;163(8):bqac103. doi: 10.1210/endocr/bqac103

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Figure 3. — Part of ARH^GHRH neurons and practically all ARH^AgRP neurons are GABAergic. A to C, Colocalization between Ghrh mRNA (magenta staining) and Slc32a1 mRNA (green staining) in the ARH (n = 4). The arrows indicate double-labeled neurons. D to F, No colocalization between Ghrh mRNA (magenta staining) and Slc17a6 mRNA (green staining) in the ARH (n = 4). G to I, ARH^VGAT neurons (GFP staining in green representing VGAT-expressing cells) colocalize with AgRP peptide (magenta staining; n = 2). J to L, ARH^VGLUT2 neurons (GFP staining in green representing VGLUT2-expressing cells) do not express AgRP (n = 2). Scale bar = 100 µm. 3V, third ventricle; AgRP, agouti-related protein; ARH, arcuate nucleus; GFP, green fluorescent protein; mRNA, messenger RNA; VGAT, vesicular inhibitory amino acid transporter; VMH, ventromedial nucleus.