Table 3.
Validation of cell-specific ablation of growth hormone receptor (GHR) in vesicular inhibitory amino acid transporter GHR knockout (KO) and vesicular glutamate transporter 2 GHR KO mice
| VGAT GHR KO mice | VGLUT2 GHR KO mice | |||
|---|---|---|---|---|
| No. of pSTAT5+ cells | No. of VGAT/pSTAT5+ cells | No. of pSTAT5+ cells | No. of VGLUT2/pSTAT5+ cells | |
| ARH | 25.7 ± 3.7 | 0.0 ± 0.0 | 134.3 ± 6.7 | 0.0 ± 0.0 |
| DMH | 297.0 ± 26.3 | 0.0 ± 0.0 | 240.6 ± 30.9 | 0.0 ± 0.0 |
| MEApd | 59.8 ± 29.1 | 0.0 ± 0.0 | 139.3 ± 5.4 | 0.0 ± 0.0 |
| PMv | 188.7 ± 10.0 | 0.0 ± 0.0 | 1.8 ± 1.0 | 0.2 ± 0.2 |
| PV | 72.0 ± 11.8 | 0.0 ± 0.0 | 5.3 ± 1.7 | 0.0 ± 0.0 |
| PVH | 296.3 ± 5.4 | 0.0 ± 0.0 | 2.7 ± 1.4 | 0.0 ± 0.0 |
| VMH | 879.0 ± 50.5 | 0.0 ± 0.0 | 4.7 ± 0.6 | 0.0 ± 0.0 |
n = 3/group.
Abbreviations: ARH, arcuate nucleus; DMH, dorsomedial nucleus of the hypothalamus; GHR, growth hormone receptor; MEApd, posterodorsal part of the medial nucleus of the amygdala; PMv, ventral premammillary nucleus; PV, periventricular nucleus; PVH, paraventricular nucleus of the hypothalamus; VGAT, vesicular inhibitory amino acid transporter; VGLUT2, vesicular glutamate transporter 2; VMH, ventromedial nucleus of the hypothalamus.