Table 2:
Accuracy (%bias) and Precision (RSD) for Quality Controls: HC, MC, LC, and LLOQ
| Analyte | QC Level | Concentration (ng/mL) | Intra-day | Inter-day | ||
|---|---|---|---|---|---|---|
| % Bias | RSD | % Bias | RSD | |||
| Midazolam | LLOQ | 0.50 | 4.2 | 5.4 | 6.0 | 5.5 |
| LC | 1.5 | −10.8 | 2.7 | −9.0 | 4.2 | |
| MC | 30 | −4.6 | 4.2 | −7.1 | 4.6 | |
| HC | 100 | −0.2 | 8.7 | −2.3 | 8.2 | |
| 1-OH MDZ | LLOQ | 5 | −0.1 | 6.1 | −0.2 | 6.3 |
| LC | 15 | −10.3 | 1.8 | −9.3 | 4.9 | |
| MC | 60 | −4.4 | 5.0 | −4.6 | 4.1 | |
| HC | 400 | −2.6 | 11.8 | −2.8 | 8.1 | |
| Dexmedetomidine | LLOQ | 0.05 | −1.0 | 9.4 | −2.7 | 7.6 |
| LC | 0.15 | −12.2 | 2.4 | −10.2 | 3.8 | |
| MC | 0.60 | 2.9 | 6.3 | −0.8 | 7.5 | |
| HC | 6.00 | −0.3 | 7.2 | −3.3 | 7.1 | |
| Morphine | LLOQ | 0.50 | 8.2 | 5.0 | 9.6 | 4.4 |
| LC | 1.50 | −11.1 | 3.1 | −9.3 | 5.8 | |
| MC | 30 | −4.9 | 2.6 | −7.6 | 4.0 | |
| HC | 100 | −3.2 | 7.2 | −4.5 | 6.7 | |
| Hydromorphone | LLOQ | 0.50 | 5.2 | 5.9 | 8.6 | 5.9 |
| LC | 1.50 | −10.3 | 4.0 | −7.7 | 5.9 | |
| MC | 30 | −2.0 | 3.6 | −4.7 | 4.3 | |
| HC | 100 | 0.3 | 6.6 | −3.2 | 7.0 | |
| M3G | LLOQ | 5 | −0.1 | 6.6 | 1.5 | 5.0 |
| LC | 15 | −13.9 | 0.9 | −12.0 | 3.6 | |
| MC | 60 | −4.2 | 2.7 | −8.3 | 4.5 | |
| HC | 400 | −2.5 | 7.0 | −5.4 | 7.1 | |
| M6G | LLOQ | 5 | 2.2 | 5.0 | 0.7 | 4.3 |
| LC | 15 | −12.1 | 2.3 | −10.6 | 3.8 | |
| MC | 60 | −2.3 | 2.7 | −5.7 | 4.2 | |
| HC | 400 | −1.9 | 7.8 | −3.5 | 7.8 | |
| Fentanyl | LLOQ | 0.05 | −0.2 | 8.3 | −2.9 | 8.5 |
| LC | 0.15 | −10.7 | 3.3 | −8.5 | 6.8 | |
| MC | 0.60 | −2.6 | 6.8 | −2.8 | 5.9 | |
| HC | 6.00 | −3.4 | 7.9 | −4.2 | 8.0 | |
| Norfentanyl | LLOQ | 0.05 | 2.6 | 8.0 | 2.2 | 6.7 |
| LC | 0.15 | −12.9 | 5.2 | −9.8 | 4.9 | |
| MC | 0.60 | 1.4 | 4.9 | −4.1 | 6.0 | |
| HC | 6.00 | 2.3 | 6.9 | −1.6 | 8.1 | |