Table 3:

Matrix effect and % Recovery for Sedatives in human plasma

Analyte	QC Level	Concentration (ng/mL)	Aqueous Sample (ng/mL)	Extracted Sample (ng/mL)	Post Extracted Sample (ng/mL)	% Matrix Effect	% Recovery
Midazolam	LC	1.5	1.3	1.4	1.3	101.6	108.0
	MC	30	27.9	26.8	27.6	98.9	97.4
	HC	100	102.9	103.1	106.1	103.2	97.3
1-OH MDZ	LC	15	15.6	14.9	16.0	102.3	93.2
	MC	60	61.7	56.2	59.0	95.7	95.6
	HC	400	424.0	405.2	437.2	103.3	92.7
Dexmedetomidine	LC	0.15	0.16	0.14	0.16	98.4	90.5
	MC	0.60	0.64	0.55	0.62	97.0	88.6
	HC	6.00	6.03	5.81	5.52	91.5	105.3
Morphine	LC	1.5	1.5	1.5	1.5	100.0	100.1
	MC	30	31.1	27.0	28.0	90.2	96.5
	HC	100	106.3	99.6	111.4	104.8	89.4
Hydromorphone	LC	1.5	1.6	1.5	1.6	95.9	96.4
	MC	30	29.8	27.0	28.8	96.8	93.8
	HC	100	107.0	96.8	100.7	94.2	96.2
M3G	LC	15	17.0	13.8	25.2	147.8	55.1
	MC	60	64.5	54.9	104.8	162.3	53.1
	HC	400	418.4	386.4	693.7	165.8	56.0
M6G	LC	15	16.6	14.3	21.3	128.0	67.6
	MC	60	67.3	53.9	97.0	144.2	55.7
	HC	400	430.0	401.5	659.3	153.4	61.0
Fentanyl	LC	0.15	0.16	0.15	0.16	100.2	93.1
	MC	0.60	0.65	0.58	0.63	96.5	92.0
	HC	6.00	6.03	6.17	5.52	91.6	111.8
Norfentanyl	LC	0.15	0.17	0.15	0.16	93.2	94.7
	MC	0.60	0.68	0.54	0.59	87.7	90.7
	HC	6.00	6.00	5.92	5.73	95.9	103.4