Table 4:
Stability for sedatives in human plasma
| Analyte | QC Level | Concentration (ng/mL) | 4-hour Bench Top | Freeze-Thaw (After 3 cycles) | Autosampler (72 hours) | |||
|---|---|---|---|---|---|---|---|---|
| % Of Target | RSD | % Of Target | RSD | % Of Target | RSD | |||
| Midazolam | LC | 1.5 | 103.1 | 7.0 | 103.3 | 5.9 | 90.5 | 2.4 |
| HC | 100 | 99.0 | 4.1 | 107.4 | 4.3 | 96.3 | 7.8 | |
| 1-OH MDZ | LC | 15 | 101.3 | 1.0 | 100.2 | 0.5 | 87.8 | 3.9 |
| HC | 400 | 101.0 | 5.1 | 105.9 | 2.6 | 100.1 | 8.8 | |
| Dexmedetomidine | LC | 0.15 | 96.4 | 6.0 | 96.9 | 3.7 | 89.6 | 3.0 |
| HC | 6.00 | 94.6 | 4.7 | 109.6 | 5.9 | 97.9 | 2.0 | |
| Morphine | LC | 1.5 | 95.0 | 8.3 | 99.6 | 5.2 | 90.0 | 1.6 |
| HC | 100 | 99.9 | 2.3 | 109.8 | 4.7 | 96.5 | 6.3 | |
| Hydromorphone | LC | 1.50 | 96.6 | 3.8 | 97.4 | 1.6 | 93.3 | 5.5 |
| HC | 100 | 102.4 | 1.8 | 109.4 | 3.8 | 98.9 | 6.3 | |
| M3G | LC | 15 | 94.2 | 3.3 | 91.7 | 0.5 | 104.2 | 1.6 |
| HC | 400 | 102.1 | 1.7 | 106.9 | 2.5 | 97.3 | 9.9 | |
| M6G | LC | 15 | 98.4 | 2.7 | 97.5 | 6.4 | 98.7 | 6.7 |
| HC | 400 | 103.5 | 1.8 | 102.9 | 8.2 | 104.0 | 7.8 | |
| Fentanyl | LC | 0.15 | 91.8 | 4.9 | 103.8 | 6.5 | 92.3 | 1.7 |
| HC | 6.00 | 98.4 | 1.7 | 109.8 | 2.9 | 99.0 | 4.2 | |
| Norfentanyl | LC | 0.15 | 88.9 | 4.4 | 96.0 | 0.4 | 87.0 | 1.4 |
| HC | 6.00 | 101.9 | 2.7 | 109.0 | 4.4 | 103.7 | 8.7 | |