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. 2022 Jul 21;12:12489. doi: 10.1038/s41598-022-14443-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The predicted ${Δ Δ G}^{Stability}$ for the m396 multi-point mutants from the FEP simulations vs. the shift of their experimentally measured melting temperature relative to the wildtype m396. Some of the horizontal error bars are smaller than the size of the data marker and are thus invisible.