Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | B6.129S-Chattm1(cre)Lowl/MwarJ | The Jackson Laboratory | Strain #:031661 RRID:IMSR_JAX:031661 |
B6J.ChAT-IRES-Cre (Δneo) |
| Genetic reagent (M. musculus) | B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J | The Jackson Laboratory | Strain #:007612 RRID:IMSR_JAX:007612 |
Thy1-ChR2-YFP |
| Genetic reagent (M. musculus) | B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J | The Jackson Laboratory | Strain #:012569 RRID:IMSR_JAX:012569 |
Ai32(RCL-ChR2(H134R)/EYFP) |
| Genetic reagent (M. musculus) | STOCK Slc17a6tm2(cre)Lowl/J | The Jackson Laboratory | Strain #:016963 RRID:IMSR_JAX:016963 |
Vglut2-ires-cre |
| Chemical compound, drug | Mecamylamine hydrochloride | Sigma-Aldrich | Lot # 019M4108V CAS: 826-39-1 |
|
| Chemical compound, drug | Atropine sulfate salt monohydrate | Sigma-Aldrich | Lot # BCBH8339V CAS No.: 5908-99-6 |
|
| Chemical compound, drug | SR 95531 hydrobromide (Gabazine) | Hello Bio | CAS: 104104-50-9 |
|
| Chemical compound, drug | DNQX | TOCRIS | CAS: 2379-57-9 |
|
| Chemical compound, drug | D-AP5 | Hello Bio | CAS:79055-68-8 | |
| Chemical compound, drug | CGP 55845 hydrochloride | Hello Bio | CAS: 149184-22-5 | |
| Chemical compound, drug | Dihydro-β-erythroidine hydrobromide | TOCRIS | CAS: 29734-68-7 | |
| Chemical compound, drug | Ranolazine dihydrochloride | Sigma-Aldrich | CAS Number: 95635-56-6 |
Product Number R6152 |
| Chemical compound, drug | ZD7288 | MedChemExpress | CAS No.: 133059-99-1 | Synonyms: ICI D7288 |
| Chemical compound, drug | Tetrodotoxin citrate | Hello Bio | CAS: 18660-81-6 | |
| Chemical compound, drug | 4-Aminopyridine | Sigma-Aldrich | CAS Number: 504-24-5 |
|
| Chemical compound, drug | Phosphate buffered saline tablets | Sigma-Aldrich | MDL number: MFCD00131855 |
Product Number P4417 |
| Chemical compound, drug | Paraformaldehyde | Sigma-Aldrich | CAS Number: 30525-89-4 |
|
| Chemical compound, drug | XYLAZINE AS HYDROCHLORIDE | EUROVET ANIMAL HEALTH B.V | CAS: 082-91-92341-00 | |
| Chemical compound, drug | CLORKETAM | VETOQUINOL | CAS: 1867-66-9 | |
| Chemical compound, drug | Meloxicam | Chanelle Pharmaceuticals Manufacturing ltd | CAS Number: 71125-38-7 |
|
| Chemical compound, drug | isoflurane | Primal Critical Care | CAS Number: 26675-46-7 |
|
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | Data analysis |
| Software, algorithm | WinWCP | University of Strathclyde Glasgow | RRID:SCR_014713 | Data acquisition |
| Software, algorithm | FEMTOSmart Software: MESc | FEMTONICS | RRID:SCR_018309 | 2 P Data acquisition |
| Software, algorithm | NIS-Elements Basic Research | Nikon instruments | RRID:SCR_002776 | Confocal images acquisition |
| Software, algorithm | Signal 6 | Cambridge Electronic Design | RRID:SCR_017081 | |
| Software, algorithm | AlphaLab SnR | Alpha-Omega Engineering | https://www.alphaomega-eng.com/ | |
| Software, algorithm | Electrode Positioning System | Alpha-Omega Engineering | https://www.alphaomega-eng.com/ | |
| Other | Model 940 Small Animal Stereotaxic Instrument with Digital Display Console | Kopf Instruments | https://kopfinstruments.com/product/model-940-small-animal-stereotaxic-instrument-with-digital-display-console/ | See “Stereotaxic viral inoculation in Vglut2-ires-Cre mice” in Materials and Methods |
| Other | Sound-attenuating room | IAC acoustics | https://www.iacacoustics.com/ | See “Non-Human Primates (NHPs)” in Materials and Methods |
| Other | Glass-coated Tungsten electrodes | Alpha-Omega Engineering | https://www.alphaomega-eng.com/ | See “Non-Human Primates (NHPs)” in Materials and Methods |