Table 3. Buffer used for recombinant Embp (rEmbp) purification.

Buffer name	Composition
Binding/lysis	50 mM K2PO4, 500 mM NaCl, 400 mM imidazole, pH 7.4
Ni-NTA elution	50 mM K2PO4, 500 mM NaCl, 40 mM imidazole, pH 7.4
IEX binding	20 mM Bis-Tris propane, pH 6.0
IEX elution	20 mM Bis-Tris propane, 1 M NaCl, pH 6.0
SEC	50 mM MES, 150 mM NaCl, pH 6.0