Table 2. Plasmids used in this study.

Plasmid	Description	Primers (5' to 3', Fw: Forward, Rev: Reverse)	Source
pEM784	nlCre recombinase	NA	Khandelia 2011 PMID 21768390
pEM791	EGFP for recombination	NA	Khandelia 2011 PMID 21768390
pJS2161	GFP-KIF22	NA	Stumpff 2012 PMID 22595673
pAT4206	KIF22-GFP	Fw: TACGTGGAATTCCACCATGGCCGCGGGCGGCTCGA Rev: GTGACTGGTACCTGGAGGCGCCACAGCGCTGGC	This study
pAT4226	KIF22-GFP, siRNA resistant	Fw:pGGGCATGGACAGCTGCTCACTCGAAATCGCTAACTGGAGGAACCAC Rev:pGTGGTTCCTCCAGTTAGCGATTTCGAGTGAGCAGCTGTCCATGCCC	This study
pAT4250	KIF22-GFP, siRNA resistant, for recombination	Fragment Fw: CTGGGCACCACCATGGCCGCG Fragment Rev: GCTAGCTCGATTACTTGTACAGCTCGTCCATGCC Vector Fw: GTACAAGTAATCGAGCTAGCATATGGATCCATATAACT Vector Rev: CATGGTGGTGCCCAGTGCCTCACGACC	This study
pAT4251	KIF22-GFP R149Q	Fw: GGGGTGATCCCGCAGGCTCTCATGGAC Rev: GTCCATGAGAGCCTGCGGGATCACCCC	This study
pAT4258	KIF22-GFP V475G	Fw: TGCTAATGAAGACAGGAGAAGAGAAGGACCT Rev: AGGTCCTTCTCTTCTCCTGTCTTCATTAGCA	This study
pAT4260	KIF22-GFP T463D	Fw: CCCCTCTGTTGAGTGACCCAAAGCGAGAGC Rev: GCTCTCGCTTTGGGTCACTCAACAGAGGGG	This study
pAT4261	KIF22-GFP T463A	Fw: CCTCTGTTGAGTGCCCCAAAGCGAG Rev: CTCGCTTTGGGGCACTCAACAGAGG	This study
pAT4264	KIF22-GFP R149L	Fw: GGGTGATCCCGCTGGCTCTCATGGAC Rev: GTCCATGAGAGCCAGCGGGATCACCC	This study
pAT4269	KIF22-GFP P148L	Fw: CCTGGGGTGATCCTGCGGGCTCTCATG Rev: CATGAGAGCCCGCAGGATCACCCCAGG	This study
pAT4270	KIF22-GFP P148S	Fw: CTGGGGTGATCTCGCGGGCTCTCATG Rev: CATGAGAGCCCGCGAGATCACCCCAG	This study
pSS4279	KIF22-GFP T134A	Fragment Fw: AGCTGCTCACTCGAAATCGC Fragment Rev: AGTCTTTCTCGGATTACCAGG Vector Fw: CCTGGTAATCCGAGAAGACT Vector Rev: GCGATTTCGAGTGAGCAGCT	This study
pSS4281	KIF22-GFP T134D	Fw: CAGGAGCTGGGAAGGATCACACAATGCTGGGC Rev: GCCCAGCATTGTGTGATCCTTCCCAGCTCCTG	This study
pNA4285	KIF22-GFP T158A	Fw: AGCTCGCAAGGGAGGAGGGTG Rev: GAGTACCTGGAGGACGTCGA	This study
pNA4284	KIF22-GFP T158D	Fw: CCTCCTGCAGCTCAGGGAGGAGGGTG Rev: CACCCTCCTCCCTGAGCTGCAGGAGG	This study
pAT4291	mCh-Tail 442–506	Fragment Fw: ccggactcagatctcgaggacgcctcctcagcttggaccg Fragment Rev: ctgattatgatcagttatctgttctccttttcctcagccttctg Vector Fw: aggctgaggaaaaggagaacagataactgatcataatcagccatac Vector Rev: cggtccaagctgaggaggcgtcctcgagatctgagtccgg	This study
pAT4292	mCh-Tail 420–520	Fragment Fw: ccggactcagatctcgaggagcctctgcctcccagaaact Fragment Rev: ctgattatgatcagttatctgactgtgcgatgtgaaaggg Vector Fw: ccctttcacatcgcacagtcagataactgatcataatcagccatac Vector Rev: agtttctgggaggcagaggctcctcgagatctgagtccgg	This study
pAT4294	Motor Domain-GFP 1–383	Fw: aggtaccgcgggcccgggat Rev: ccaatgagagcctgcagcctcatgccttg	This study