Fig. 3.

Effects of compound C on THC attenuated lipogenesis and AMPK signaling in OA-treated HepG2 cells. HepG2 cells were pretreated 2 μM compound C for 1 h followed by stimulation of OA for 12 h, and then treated with 10–100 μM of THC for another 24 h. (A) Oil Red O stained HepG2 cells were photographed ( × 200 magnification) and (B) lipid content was extracted and measured by spectrophotometric analysis at 520 nm. Protein extracts were prepared and the protein levels of (C) p-AMPKα (Thr172), AMPKα, p-ACC (Ser79) and ACC, (D) SREBP-1c and FAS were examined by Western Blot analysis. Data were presented as the mean ± SE (n = 3). Means with different letters (a–d) above the bars are significantly different (P < 0.05).