Table 1.
Linearity, LOQ, and recovery rate of four aflatoxins in Astragli Radix by HPLC.
| Linearity | ||||
|---|---|---|---|---|
|
| ||||
| Items | Concentration range (mg/kg) | Linear regression equation | R2 | |
| AFB1 | 0.309375e19.8 | Y = 83270X + 2748 | 0.99999 | |
| AFB2 | 0.095625e6.12 | Y = 181589X + 1490 | 0.99999 | |
| AFG1 | 0.54375e17.4 | Y = 34305X + 385 | 0.99994 | |
| AFG2 | 0.1875e6 | Y = 72147X + 3185 | 0.99871 | |
|
| ||||
| LOQ | ||||
|
| ||||
| Items | Spike value (mg/kg) | Detection value (mg/kg) | S/N ratio | S/N = 10 (mg/kg) |
|
| ||||
| AFB1 | 0.1547 | 0.1869 | 9.08 | 0.20 |
| AFB2 | 0.0956 | 0.1060 | 12.35 | 0.10 |
| AFG1 | 0.5438 | 0.5738 | 14.73 | 0.40 |
| AFG2 | 0.1875 | 0.1857 | 12.58 | 0.15 |
|
| ||||
| Recovery rate | ||||
|
| ||||
| Items | Spike value (mg/kg) | Detection value (mg/kg) | Recovery rate (%)a | |
|
| ||||
| AFB1 | 0.309375 | 0.2922 ± 0.02 | 94.45% | |
| 2.475 | 2.5072 ± 0.05 | 101.30% | ||
| 19.8 | 20.2128 ± 0.30 | 102.08% | ||
| AFB2 | 0.095625 | 0.0953 ± 0.01 | 99.66% | |
| 0.765 | 0.7853 ± 0.03 | 102.65% | ||
| 6.12 | 6.2072 ± 0.40 | 101.42% | ||
| AFG1 | 0.54375 | 0.5612 ± 0.03 | 103.21% | |
| 2.175 | 2.1856 ± 0.07 | 100.49% | ||
| 17.4 | 17.2867 ± 0.48 | 99.35% | ||
| AFG2 | 0.1875 | 0.1640 ± 0.02 | 87.47% | |
| 0.75 | 0.7519 ± 0.01 | 100.25% | ||
| 6 | 5.8828 ± 0.18 | 98.05% | ||
a
The recovery rate was calculated as follows: (detection of value ÷ spike concentration) × 100%.