Fig. 3.

c-Myc regulates ribosomal protein S19 (RPS19) expression. A. A431-P cells were transfected with 1 μg of pcDNA3-HA as the control, and 0.5 and 1.0 μg of pcDNA3-cMyc-HA. Protein levels of RPS19 was measured by Western blotting after 24 h. B. Protein level changes of RPS19 after transfection with the control or pcDNA3-cMyc-HA were analyzed with ImageJ software. C. A431-III cells were treated with DMSO, or 100, 200, and 400 μM of 10058-F4 for 24 h, and protein levels of RPS19 were measured by Western blotting. D. Protein level changes of RPS19 after treatment with DMSO or 10058-F4 were analyzed by ImageJ software. Data represent the mean (SD) of three different experiments. Statistical significance was determined by a one-way ANOVA with Tukey's test (*P < 0.05, **P < 0.01, and ***P < 0.001).