Fig. 4.

cMyc activates transactivation of ribosomal protein S19 (RPS19) in A431-P cells. A. The RPS19 promoter contains six potential E-box motifs. B. Transactivation of RPS19 was measured by a luciferase assay after transfection of A431-P cells with pcDNA3-HA (control), or 0.5 and 1.0 μg of pcDNA3-cMyc-HA for 24 h in A431-P cells. C. Transactivation of RPS19 was measured by transfection of A431-III cells with pcDNA3-HA (control), 0.25 and 0.5 μg of pcDNA3-cMyc-HA, and combined treated with or without 10058-F4 A431-III cells for 24 h in A431-III cells. Statistical significance between the groups was determined from three separate experiments by an unpaired Student's t-test. Results from three independent experiments are expressed as the mean ± standard deviation (SD). Statistical significance was determined by a one-way ANOVA with Tukey's test (*P < 0.05, **P < 0.01, and ***P < 0.001). that RPS19 expression might be reduced by both Lu and Qu through the Akt/mTOR signaling pathway.