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. 2022 Jul 22;847:157547. doi: 10.1016/j.scitotenv.2022.157547

Table 1.

Primer and probe sequences used for ddPCR.

Target Primer/probe Sequence Reference
SARS-CoV-2 2019-nCoV_N1-F 5′-GACCCCAAAATCAGCGAAAT-3′ Lu et al. (2020)
2019-nCoV_N1-R 5′-TCTGGTTACTGCCAGTTGAATCTG-3′
2019-nCoV_N1-P 5′-FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1–3′
2019-nCoV_N2-F 5′-TTACAAACATTGGCCGCAAA-3′
2019-nCoV_N2-R 5′-GCGCGACATTCCGAAGAA-3′
2019-nCoV_N2-P 5′-HEX-ACAATTTGCCCCCAGCGCTTCAG-BHQ1-3′
Phi6 Phi6-F 5′-TGGCGGCGGTCAAGAGC-3′ Gendron et al. (2010)
Phi6-R 5′-GGATGATTCTCCAGAAGCTGCTG-3′
Phi6-P 5′-6FAM-TCCGCCTGGCACGGTACTCCCT-BHQ1-3′