Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 8;50(13):7783–7799. doi: 10.1093/nar/gkac587

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — MultiMate enables rapid modular assembly of multifunctional DNA circuitry for efficient baculovirus-vectored delivery in human cells. (A) MultiMate assembly platform in a schematic view. Symbols are listed (left panel). attL/R flanked DNA from ENTR plasmid modules (upper box, dashed) are assembled on MultiBac-DEST (lower box) and further combined by in vitro Cre-mediated recombination to generate multicomponent MultiMate plasmids, maximally eliminating prokaryotic backbone DNA sequences. MultiMate plasmids are integrated in BVs customized for efficient delivery in human cells (right panel). (B) Confocal live cell imaging of H4, HeLa, HEK293T and SH-SY5Y 48 hours after transduction with MultiMate-Rainbow BV (upper panel) evidencing in all cells homogeneous sustained expression and correct subcellular localization of H2B-iRFP (nucleus), GTS-mTagBFP (Golgi), mAG-β-catenin (membrane and adherens junctions), EYFP-Tubulin (microtubules), mTFP1-Actin (cytoskeleton), mito-mCherry (mitochondria) and CyOFP1-ER (endoplasmic reticulum). Scalebar, 20 μm. (C) Twelve-hours confocal time-lapse imaging of live HeLa cells transduced with MultiMate-CellCycle BV (snapshots, Supplementary Video 1). Transduced cells constitutively express H2B-iRFP for DNA imaging. mAG-hGeminin and mKO2-hCdt1 are stabilized in S/G2 and G1 cell cycle stages, respectively. Upper panel shows G1/S transition, lower panel shows a dividing cell (arrows indicate tracked cells). Scalebar, 10 μm. DNA elements, plasmid topology and cell cycle schematics are illustrated (upper panel).