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. 2022 Feb 25;50(13):7570–7590. doi: 10.1093/nar/gkac116

Figure 5.

Figure 5.

Overexpression of MiaA reduces ExPEC stress resistance. (A) Relative levels of i6A and ms2i6A in UTI89 carrying either pMiaAPtac or the empty vector control pRR48 following growth to OD600 of 0.5 in LB with 1 mM IPTG, as quantified by LC-MS. ****P < 0.0001 by unpaired t test; n = 5 to 6 independent replicates per group. (B–F) Curves depict growth of UTI89 carrying pMiaAPtac or the empty vector control pRR48 in LB, LB + 5% NaCl, LB + 1mM MV, MES-LB, or MES-LB + 1 mM ASN. Cultures were grown shaking at 37°C with IPTG added in 10-fold increments from 0 to 1000 μM, as indicated. Curves depict mean values from four replicates and are representative of three independent experiments.