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. 2022 Feb 25;50(13):7570–7590. doi: 10.1093/nar/gkac116

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Conditional adverse effects of increased DMAPP binding by MiaA. (A) Crystal structure-based model of MiaA (grey) complexed with tRNA (pink) and DMAPP (blue). The three residues that were mutated to alanines are indicated. Image created using PyMOL with structural information (2ZXU) downloaded from the RCSB Protein Data Bank (125). (B) Graphs show growth curves for recombinant UTI89 strains with the specified plasmids in (i) LB with 5% NaCl (left) or (ii) 1 mM MV (right) ±1 mM IPTG, as indicated. (C) Growth curves for UTI89/pMiaA_P_tac in (i) LB with 5% NaCl (left) or (ii) 1 mM MV (right) ± HDMAPP and IPTG, as noted. Each curve shows average values from four replicates and are representative of three independent experiments. (D) A working model of how changing levels of MiaA can impact translational fidelity and stress resistance. Created with Biorender.com.