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Characterisation and coupling yield of the post-synthetically modified DNA strands with bioconjugation handles
| Oligo product | Mass calculated (Da) | Mass found (Da) | Conjugation yield (%)a |
|---|---|---|---|
| 9a | 6258.3 | 6257.7 | Quant. |
| 9c | 6455.6 | 6457.7 | 62b & Quant.c |
| 9d | 7143.2 | 7143.1 | Quant. |
| 9e | 6570.5 | 6570.4 | 85 |
| 9f | 6571.7 | 6571.5 | 36 |
aYield calculated using the chromatogram area of each peak.
bYield obtained in deionised water solution.
cYield obtained in a 1:1 MQ:DMSO solution.
