Figure 8.

Progression curves of 100‐fold Jump‐Dilution experiments at HDAC1. Inhibitor concentrations are indicated on the right. Vorinostat and entinostat are included as reference inhibitors. Fluorescence of cleaved AMC is measured in relative fluorescence units (RFU). When compared to vehicle the final slope of the respective progression curves roughly indicates the amount of free enzyme. Final slope [RFU*min⁻¹]: vehicle [DMSO 1 %]=111.9; vorinostat [0.011 μM]=105.0; entinostat [0.011 μM]=78.4. A) Jump‐Dilution experiment of 6 d, final slope [RFU*min⁻¹]: 6 d [0.004 μM]=39.4; B) Jump‐Dilution experiment of 10 c, final slope [RFU*min⁻¹]: 10 c [0.0025 μM]=26.2. Experiments were performed in triplicates.