Figure 10.

Biological evaluation of 6 d, and 10 c compared to the reference inhibitors vorinostat and entinostat. A) Target assessment by western blot. Direct target analysis of cell lysates after 24‐hour treatment with the indicated concentrations of the HDACi used. B) Cell migration kinetics of MCF‐7 breast cancer cells in a modified wound healing assay in the presence of selected HDACi. Time‐dependent wound closure of cell layer after 20 h, 48 h, and 97 h indicate an inhibitory efficiency of both 10 c and 6 d. C) Wound closure after 97 h compared to the respective control (t=0 h). Significances were determined by the Dunnett's test (n=3) and are indicated by asterisks (ns, not significant; *p<0.05; **p<0.01; ***p<0.001).