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. 2022 Jul 8;50(13):7224–7234. doi: 10.1093/nar/gkac562

Table 1.

TS-ASO sequences obtained from the hepatotoxicity screeninga

Number of motifs
Name Sequence TGC TCC AST (Ul–1) ALT (Ul–1)
TS1-ASO GTTATGCCACCCTA 1 0 9437 15 602
TS2-ASO GTCCGCATGCCTAA 1 1 1364 1617
TS3-ASO GATATGCCCTACTA 1 0 448 1061
TS4-ASO GTATGCCTCCGTTA 1 1 2164 600
TS5-ASO GCTATGTTAGTCCG 0 1 287 291

aAll oligonucleotides were fully modified with phosphorothioate linkages. Gap DNA and LNA are indicated in black and green, respectively. C57BL/6J mice (n = 2 animals per group) were intravenously injected with a dose of 20 mg/kg. Ninety-six hours post injection, serum aspartate aminotransferase (AST)/alanine aminotransferase (ALT) levels were determined.