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. 2022 Feb 10;15(9):e202101062. doi: 10.1002/cssc.202101062

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© 2021 The Authors. ChemSusChem published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Characterization of PHL enzymes: (A) Phylogenetic tree of PHL and other confirmed or putative polyester hydrolases from Actinobacteria with reference to the metagenomic LCC and Ideonella sakaiensis. (B) Weight loss of amorphous PET film determined after a reaction time of 24 h at different temperatures with PHL1, PHL2, PHL4, PHL5, and PHL6 compared to TfCut2. (C) Weight loss of amorphous PET films determined after a reaction time of 24 h at different temperatures with PHL3 and PHL7 compared to TfCut2. (D) Relative weight loss of amorphous PET films determined after a reaction time of 24 h at 70 °C with PHL3, PHL7 (100 %), and the corresponding variants F210L and L210F.