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. 2022 Jul 12;50(13):7721–7738. doi: 10.1093/nar/gkac577

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — (A) The κ angle defines the orientation of the 2′-hydroxyl group, with HO2′ directed toward base (b, goldenrod), phosphate (p, purple) or sugar (s, green). Please see also similar torsion angle definitions put forth by others (17–19). (B) Sequence of SRL RNA. The underlined 12mer is universally conserved and the tetraloop is highlighted in italicized font. (C) Secondary structure of SRL RNA. Residue numbers correspond to E. coli 23S rRNA and residues are colored according to the orientation of their 2′-OH groups as defined in panel A and established by neutron crystallography. Throughout the text and in the PDB coordinate file, SRL nucleotides are numbered (U)47 to (G)73.