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Phosphate hydration involving eight nucleotides including the tetraloop G59−A60−G61−A62. Water molecules link adjacent OP2 oxygens and then cross the major groove, whereby phosphate groups of U56 and A62 serve as bridgeheads. Water molecules (O red, D cyan) and OP2 oxygens are highlighted in ball-and-stick mode, residues are labeled and H-bonds are drawn as thin solid lines. A somewhat long distance between OP1 of G61 and the nearest water is indicated by a dashed line.
