Table 1.
Selected crystal data, diffraction data collection and X-ray/neutron joint refinement statistics
| Diffraction method | SRL Cryo X-ray data | SRL Cryo neutron data |
|---|---|---|
| Wavelength [Å] | 1.342 | 2.0–4.0 |
| Resolution range [Å] | 16.05–0.998 (1.033- 0.998) | 12.34–2.25 (2.33–2.25) |
| Space group | P43 | |
| Unit cell: a= b, c [Å] | 29.495, 76.52 | |
| Total reflections | 99 309 | 9850 (771) |
| Unique reflections | 35 326 (3,461) | 2887 (280) |
| T min/Tmax | 0.5940/0.7488 | |
| Multiplicity | 13.68 (10.96) | 3.40 (2.73) |
| Completeness [%] | 99.67 (98.30) | 92.32 (92.41) |
| Mean I/sigma(I) | 21.66 (4.45) | 9.8 (2.8) |
| Wilson B-factor [Å2] | 8.30 | 25.34 |
| R-merge | 0.055 (0.412) | 0.212 (0.299) |
| R-meas | 0.029 (0.404) | 0.247 (0.357) |
| R-pim | 0.020 (0.186) | 0.121 (0.189) |
| CC1/2 | 0.999 (0.849) | 0.886 (0.300) |
| Reflections in refinement | 35 272 (3,456) | 2886 (280) |
| Reflections for R-free | 1760 (174) | 143 (13) |
| R-work | 0.1785 (0.2452) | 0.2447 (0.3310) |
| R-free | 0.1965 (0.2838) | 0.3079 (0.3171) |
| Number of non-H atoms | 709 | |
| RNA | 624 | |
| ligands | 5 | |
| solvent | 240 | |
| RMSD bonds [Å] | 0.014 | 0.090 |
| RMSD angles [°] | 1.78 | 1.61 |
| Clashscore | 2.11 | 42.39 |
| Average B-factor [Å2] | 17.2 | |
| ligands | 61.1 | |
| solvent | 31.3 | |
| PDB ID code | 7UCR | |
Statistics for the highest-resolution shell are shown in parentheses.