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. 2022 Jul 8;50(13):7298–7309. doi: 10.1093/nar/gkac593

Figure 5.

Figure 5.

C/EBPγ-ATF4 heterodimers functions as key transcriptional activators for amino acid starvation-induced autophagy. (A) Co-immunoprecipitation assay was performed to detect the interaction between ATF4 and C/EBPγ in MEFs upon amino acid starvation and glucose starvation. (B) ChIP assays were performed using anti-C/EBPγ, anti-ATF4 and anti-C/EBPβ antibodies on CARE of Map1lc3b, Bnip3, Atg12, Gadd45b, and Vps26a genes in MEFs by knocking down with either siNS or siC/EBPγ in the absence or presence of amino acid starvation. Bars, mean ± s.e.m.; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, NS non-significant. Statistics by two-tailed t-test. (C) ChIP assays were performed using anti-C/EBPγ, anti-ATF4 and anti-C/EBPβ antibodies on CARE of Map1lc3b, Bnip3, Atg12, Gadd45b and Vps26a genes in MEFs by knocking down with either siNS or siC/EBPγ in the absence or presence of glucose starvation.