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. 2022 Jun 29;50(13):7350–7366. doi: 10.1093/nar/gkac541

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — H3.3B localized on the sex chromosomes from the middle pachytene to round spermatids during spermatogenesis. (A, B) Immunostaining of spermatogenic cells from meiosis (A) to post-meiosis (B). Cells were triple-stained on WT testis section with anti-H3.3, anti-γH2AX, anti-SCP3 (A) or anti-PNA (B) as well as DAPI. Developmental stages were classified by staining profiles of SCP3, γH2AX and PNA. Scale bars, 8 μm (a46), 3 μm (a47) and 8 μm (b12). The asterix represents the sex chromosome either x or y (b4, b8, b12).