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Isoforms of representative S. oralis proteins. Cellular proteins derived from cells cultured at pH 5.2 or pH 7.0 were resolved by 2-D PAGE. (A) Glyceraldehyde-3-phosphate dehydrogenase; (B) pyruvate oxidase; (C) 60-kDa chaperonin. Individual isoforms were identified by peptide mass fingerprinting and comparison with the S. pneumoniae genomic database.
