Figure 5.

In vivo antibiofilm performance of Ni@Co‐NC. a) Schematic illustration of the treatment schedule for in vivo antibiofilm experiment. b) Traces of wound closure over 12 days for groups treated with (I) PBS, (II) vancomycin, (III) H₂O₂, and (IV) Ni@Co‐NC+laser+H₂O₂. c) Histologic analysis of the wounds treated by (I)–(IV) after 12 days of therapy. Black scale bar is 50 μm. In this experiment, we used H&E, Masson, and CD31 staining, respectively, to study inflammation response, collagen deposition, and revascularization in the wound area. d) CD31 and DAPI staining images of 3D reconstruction and corresponding maximum intensity projection (MaxIP) for healthy tissue and wounds treated by (I)–(IV). White scale bar is 50 μm. e) Inflammatory infiltration fraction, f) collagen volume fraction, g) CD31 expression, and h) CD31 positive vessels number of wounds treated by (I)–(IV) after 12 days of therapy. Asterisks indicate significant differences (*P<0.1, **P<0.01, ***P<0.001). All data were acquired from the rabbits’ wound tissues in different groups 12 days after treatment. All values are expressed as mean±SD, n=3.