Figure 1.

Schematic illustrating protein recruitment and release from coacervate‐based artificial cells using DNA strand displacement. A) Liquid–liquid phase separation of oppositely charged cationic quaternized amylose (Q‐am) and anionic carboxymethyl‐amylose (CM‐am) into microdroplets. B) Hierarchical self‐assembly of the terpolymer (PEG‐PCLgTMC‐PGlu) on the droplet surface. C) Preparation of protein–DNA conjugates via BCN–azide click chemistry. The UPT strand was attached to connected‐DNA on proteins. Cy3 and Cy5 dyes were covalently introduced to Mb and HRP, respectively, before introduction of UPT strand. D) Exemplary sequestration of eYFP in the coacervate core following hybridization to a complementary DNA uptake (UPT) strand. E) Release of eYFP from the coacervate matrix triggered by the addition of a displacing releaser (REL) DNA strand.