Figure 3.

Multiplex control over protein release from coacervate‐based artificial cells. A) Schematic and quantification of the release of 1 equivalent eYFP (100 nM) from coacervate‐based artificial cells following two times addition of 0.5 equivalents REL strand (50 nM) at 20 and 70 minutes (black arrowheads). Graph depicts the decrease in eYFP fluorescence intensity inside coacervates over time. B) Dependence of REL strand complementarity on releasing speed of eYFP. Graph depicts the decrease in fluorescence intensity inside coacervates over time, following the addition of REL strands with complementary bases varying between 12+0 nt (0) and 12+20 nt (20) at room temperature. Fluorescence intensity was determined inside the core of ≥5 coacervates. Error bars represent standard deviation. For uptaking eYFP with each UPT strands, all samples were incubated over 30 minutes before adding each REL strands at 4 °C. C) Schematic and confocal images showing the selective step‐wise release of Mb, HRP and eYFP following the consecutive addition of corresponding REL strands. After adding each REL strand, the solutions were incubated for 30 minutes at room temperature. All experiments were performed in PBS containing 5 mM MgCl₂, pH 7.4, ionic strength (I)=185 mM. Different time points represent different artificial cells. Scale bars represent 20 μm.