Table 2. Preparing diluted phage library .
Component | Stock concentration | Final concentration | 110 rxns |
---|---|---|---|
VirScan phage library | 9.9 × 10 10 pfu/mL (this may vary by batch) | 2 × 10 10 pfu/mL | 22.2 mL |
chloramphenicol | 50 mg/mL (1,000×) | 50 μg/mL (1×) | 110 μL |
kanamycin | 50 mg/mL (1,000×) | 50 μg/mL (1×) | 110 μL |
Phage extraction buffer | To 110 mL |