| Primer name | Primer sequence (5’ – 3’) |
| IS7 | ACACTCTTTCCCTACACGACTCCAGTCAGGTGTGATGCTC |
| IS8 | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCCGAGCTTATCGTCGTCATCC |
| IS4 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACTCCAGT |
| Index primer | CAAGCAGAAGACGGCATACGAGATNNNNNNNGTGACTGGAGTTCAGACGTGT |
| 5’NEST-qPCR | TCGGGGATCCAGGAATTC |
| 3’NEST-qPCR | CGTCGTCATCCTTGTAATCG |
| NEST_probe | /56-FAM/TAATCGCGG/ZEN/CCGCAAGCTTGTC/3IABkFQ/ |
| T7-Illumina-READ1-A | TGCTCGGGGATCCAGGAATTCCGCTGCGT |
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