Component	Stock concentration	Final concentration	1 rxn (μL)	110 rxns (μL)
Sterile water			2.68	294.8
Reaction Buffer	5×	1×	6.0	660
dNTPs	10 mM	0.3 mM	0.90	99
Primer IS7	100 μM	0.2 μM	0.06	6.6
Primer IS8	100 μM	0.2 μM	0.06	6.6
Q5	2 U/μL	0.02 U/μL	0.30	33
Template	2×	1×	20
Total			30 μL