Component	Stock concentration	Final concentration	1 rxn (μL)	110 rxns (μL)
Sterile water			4.55	500.5
Reaction Buffer	5×	1×	2.0	220
dNTPs	10 mM	0.3 mM	0.3	33
Primer IS4	100 μM	0.5 μM	0.05	5.5
Index primer	2.5 μM	0.5 μM	2.0
Q5	2 U/μL	0.02 U/μL	0.1	11
Template	2×	1×	1.0
Total			10 μL