| STEP | TEMP | TIME |
|---|---|---|
| Initial Denaturation | 98°C | 30 s |
| Eight cycles total | 98°C | 5 s |
| 68°C | 10 s | |
| 72°C | 30 s | |
| Final Extension | 72°C | 2 min |
| Hold | 4–10°C |
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