1. Resource Table.

Unique stem cell identifier	FDCHDPe010-A-56
Alternative name(s) of stem cell line	LRP2-KO hESCs
Institution	Eye & ENT Hospital, Shanghai Medical School, Fudan University, Shanghai, China
Contact information of distributor	Jie You, 513yj45@163.com;
	Ling Chen, linglingchen98@hotmail.com
Type of cell line	ESC
Origin	Human
Additional origin info	Age: blastocyst stage Sex: female, 46, XX Ethnicity: N/A
Cell source	N/A
Clonality	Clonal
Method of reprogramming	N/A
Cell culture system used	mTeSR™1
Genetic modification	Yes
Type of modification	Induced mutation
Associated disease	Endocytosis deficiency disease
Gene/locus	Gene:LRP2 Locus:2q31.1
Method of modification	CRISPR/Cas9
Site-specific nuclease (SSN) delivery method	Plasmid transfection
All genetic material introduced into the cells	Cas plasmid
	Karyotyping
Analysis of the nuclease- targeted allele status
Method of the off-target nuclease activity surveillance	Targeted PCR/sequencing
Name of transgene or resistance	N/A
Inducible/constitutive system	Transient expression of Sacas9 and GFP under CMV promoter
Data archived/stock date	December 2020
Cell line repository/bank	N/A
Ethical approval	This study was approved by the ethics committee of Fudan University affiliated Eye & ENT Hospital (KJ2011-04)